What are the Sample Limitations

While these analyzers are fairly tolerant of sample impurities, there are some common contaminants (urea, detergents, high concentrations of buffer/salts, etc.) which are incompatible with ion-exchange chromatography. Samples in small volumes (~200 ul) and/or low concentrations (~50 mM) of simple buffers are generally suitable for analysis. Acrylamide contaminants must be removed from samples purified on PAGE by electroblotting or electroelution. Most biological buffers and detergents, even at low concentrations, will interfere with AAA. We strongly encourage consultation with the MSF staff prior to submitting any sample.

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Category: Amino Acid Analysis

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