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Peptide Sequencing by MS/MS
 

 

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Protein / Proteome Analysis

 • 2-D Gel Electrophoresis  • Amino Acid Analysis  • Mass Spectrometry (MS)  • MS Based Protein Identification  • Protein Sequencing by Edman  • Peptide Sequencing by MS/MS

DNA Services

 • Oligonucleotide Synthesis

Small Molecule Analysis

 • Mass Spectrometry

 

 

 

 

 

 

 

 

 

 

De-novo sequencing of peptides by MS/MS is the most powerful and convincing way for protein identification and characterization because: a.) no database-search engine (i.e., peptide mass mapping, Sequest/Mascot) is 100 % reliable in identifying proteins; often they need to be confirmed by interpreted peptide sequences, b.) not all proteins are found in databases and c.) real data mining to extract biological information can be done with actual sequences. Our QSTAR hybrid mass spectrometer is dedicated for high sensitivity sequencing.

References

  • Papayannopoulos, I. A. (1995) The interpretation of collision-induced dissociation tandem mass Spectra peptides. Mass Spectrometry Reviews 14, 49-73
  • Shevchenko, A., Wilm, M., Vorm, O. and Mann, M (1996) Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68, 850-858